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celltrace violet (ctv) cell proliferation kit  (Thermo Fisher)


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    Thermo Fisher celltrace violet (ctv) cell proliferation kit
    Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with <t>CellTrace</t> Violet <t>(CTV)</t> and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and <t>proliferation</t> and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).
    Celltrace Violet (Ctv) Cell Proliferation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+violet+cell+proliferation+kit+ctv/celltrace+violet/pmc12168395-73-16-22
    Average 90 stars, based on 1 article reviews
    celltrace violet (ctv) cell proliferation kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Selective expansion and differentiation of antigen-specific CD4 + T-helper cells by engineered extracellular vesicles"

    Article Title: Selective expansion and differentiation of antigen-specific CD4 + T-helper cells by engineered extracellular vesicles

    Journal: Drug Delivery

    doi: 10.1080/10717544.2025.2509969

    Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with CellTrace Violet (CTV) and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).
    Figure Legend Snippet: Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with CellTrace Violet (CTV) and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).

    Techniques Used: In Vitro, Transgenic Assay, Labeling, Control, Cell Culture, Flow Cytometry, Expressing

    Related Articles

    Cytotoxicity Assay:

    Article Title: Nivolumab augments antitumor activity of invariant natural killer T cells
    Article Snippet: .. Cytotoxicity assay of NKT cells The K562 and A549 cancer cell lines were labeled with an Invitrogen CellTrace Violet Cell Proliferation Kit (CTV) (Thermo Fisher Scienti c), in accordance with the manufacturer’s protocol. .. NKT cells preincubated with 10 μg/mL nivolumab or 10 μg/mL hIgG4 isotype control for 30 min were co-cultured with the cancer cell lines at E/T ratios of 2.5:1, 5:1, and 10:1 in 96-well plates for 4-6 h. The cells were then stained with AnnexinV-FITC and PI using an Invitrogen Dead Cell Apoptosis Kit (Thermo Fisher Scienti c), according to the manufacturer’s protocol.

    Labeling:

    Article Title: Nivolumab augments antitumor activity of invariant natural killer T cells
    Article Snippet: .. Cytotoxicity assay of NKT cells The K562 and A549 cancer cell lines were labeled with an Invitrogen CellTrace Violet Cell Proliferation Kit (CTV) (Thermo Fisher Scienti c), in accordance with the manufacturer’s protocol. .. NKT cells preincubated with 10 μg/mL nivolumab or 10 μg/mL hIgG4 isotype control for 30 min were co-cultured with the cancer cell lines at E/T ratios of 2.5:1, 5:1, and 10:1 in 96-well plates for 4-6 h. The cells were then stained with AnnexinV-FITC and PI using an Invitrogen Dead Cell Apoptosis Kit (Thermo Fisher Scienti c), according to the manufacturer’s protocol.

    Article Title: Hippo pathway-related genes expression is deregulated in myeloproliferative neoplasms.
    Article Snippet: .. To quantify cell proliferation rate, HEL.92.1.7 cells were labeled using the CellTrace Violet Cell Proliferation Kit (CTV—ThermoFisher Scientific). ..

    Article Title: The NF-κB RelA transcription factor is not required for CD8+ T-cell function in acute viral infection and cancer
    Article Snippet: Following sorting, human CD8 + T cells were left to rest for 2 days in complete RPMI with IL-2 (Proleukin, Novartis Pharma, 50 U/mL). .. Cells were then labeled using the CellTrace Violet Cell Proliferation Kit (CTV; Thermo Fisher Scientific, catalog #C34557A) and 2x10 4 cells were stimulated with Dynabeads anti-CD3/CD28 (1 bead:4 T cells, Thermo Fisher Scientific) in complete RPMI with IL-2 (25 U/mL). ..

    Article Title: NF-κB subunits RelA and c-Rel selectively control CD4+ T cell function in multiple sclerosis and cancer.
    Article Snippet: Naive CD4+ T cells were negatively isolated using the Naive CD4+ T cell Isolation Kit (Miltenyi Biotec). .. Cells were labeled using the CellTrace Violet Cell Proliferation Kit (CTV; Thermo Fisher Scientific). .. 3 × 104 cells were stimulated with different doses of T cell–depleted mitomycin C–treated Ly5.1 splenocytes and soluble anti-CD3ε (clone 145-2C11; BioXcell): 6 × 104 splenocytes + 2 μg/ml anti-CD3ε (high stim), 1.5 × 104 splenocytes + 0.5 μg/ml anti-CD3ε (medium stim), or 0.5 × 104 splenocytes + 0.2 μg/ml anti-CD3ε (low stim), in complete IMDM (Thermo Fisher Scientific, 10% FBS, 25,000 U Penicillin/Streptomycin, 10 mM sodium pyruvate, non-essential amino acids, and 50 μM 2-Mercaptoethanol).

    Staining:

    Article Title: An iron-rich subset of macrophages promotes tumor growth through a Bach1-Ednrb axis.
    Article Snippet: Ian W. Folkert1,2,3,4* , William A. Molina Arocho2,5* , Tsun Ki Jerrick To2,5 , Samir Devalaraja2,5 , Irene S. Molina2,5 , Jason Shoush2,5 , Hesham Mohei2,5 , Li Zhai5 , Md Naushad Akhtar17 , Veena Kochat7 , Emre Arslan7 , Alexander J. Lazar7,8 , Khalida Wani9 , William P. Israel7 , Zhan Zhang10 , Venkata S. Chaluvadi5 , Robert J. Norgard6 , Ying Liu5 , Ashley M. Fuller5 , Mai T. Dang5,11,12 , Robert E. Roses3 , Giorgos C. Karakousis3 , John T. Miura3 , Douglas L. Fraker3 , T.S.. Karin Eisinger-Mathason5 , M. Celeste Simon1,13 , Kristy Weber14 , Kai Tan15,16 , Yi Fan17 , Kunal Rai7 , and Malay Haldar1,2,5

    Activation Assay:

    Article Title: An iron-rich subset of macrophages promotes tumor growth through a Bach1-Ednrb axis.
    Article Snippet: Ian W. Folkert1,2,3,4* , William A. Molina Arocho2,5* , Tsun Ki Jerrick To2,5 , Samir Devalaraja2,5 , Irene S. Molina2,5 , Jason Shoush2,5 , Hesham Mohei2,5 , Li Zhai5 , Md Naushad Akhtar17 , Veena Kochat7 , Emre Arslan7 , Alexander J. Lazar7,8 , Khalida Wani9 , William P. Israel7 , Zhan Zhang10 , Venkata S. Chaluvadi5 , Robert J. Norgard6 , Ying Liu5 , Ashley M. Fuller5 , Mai T. Dang5,11,12 , Robert E. Roses3 , Giorgos C. Karakousis3 , John T. Miura3 , Douglas L. Fraker3 , T.S.. Karin Eisinger-Mathason5 , M. Celeste Simon1,13 , Kristy Weber14 , Kai Tan15,16 , Yi Fan17 , Kunal Rai7 , and Malay Haldar1,2,5



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    Image Search Results


    Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with CellTrace Violet (CTV) and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).

    Journal: Drug Delivery

    Article Title: Selective expansion and differentiation of antigen-specific CD4 + T-helper cells by engineered extracellular vesicles

    doi: 10.1080/10717544.2025.2509969

    Figure Lengend Snippet: Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with CellTrace Violet (CTV) and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).

    Article Snippet: Lymph node T cells isolated from C57BL/6 and OT-II transgenic mice were stained with 1 μM CellTrace Violet (CTV) Cell Proliferation Kit (Thermo Fisher Scientific, Waltham, MA) for flow cytometry and incubated at 37 °C for 3 min.

    Techniques: In Vitro, Transgenic Assay, Labeling, Control, Cell Culture, Flow Cytometry, Expressing

    (A) C57BL/6 mice (n=4 for each day) were injected intraperitoneally with 200ug of Poly (I:C) and sacrificed in kinetic. The percentage of Sca1+, LAG3+ and CD69+ splenic NK cells was analyzed by flow cytometry. (B) The percentage of LAG3+ among Sca1+ and Sca1-NK cells on Day2 was analyzed by flow cytometry (n=5). (C-G) C57BL/6 mice (n=2 for each experiment) were injected intraperitoneally with 200ug of Poly (I:C) and sacrificed on Day2. Splenic NK cells were stained for the phospho-epitope pS6 Ser235/236 (C) and for pAkt S473 (D). Overlays of representative histograms are shown (left panels). The MFI values are indicated on the graph and represented on the right panel for each individual, n=6, 3 experiments in total. (E) NK cells were stained with Mitotracker Green and CellRox. The MFI values for each mouse (n=7, 3 experiments in total) for the analyzed marker are represented. (F) The MFI of CD98 (left) or CD71 (right) was determined by flow cytometry on LAG3+ and LAG3-NK cells (n=3). Due to the small sample size, we did not calculate p-values. (G) SSC-A and FSC-A parameters on NK cells were measured by flow cytometry in LAG3- and LAG3+ NK cells (n=5). (H) Splenic NK cells from C57BL/6 mice (n=6, 3 experiments) were isolated, stained with Cell Trace Violet (CTV) and cultured with 100ng/mL of IL-15 for 3 days. The MFI of CTV was analyzed by flow cytometry and the overlays of representative histograms are shown (left panel). The percentage of divided NK cells for each individual was calculated using the proliferation modeling tool on the FlowJo software. All the data were analyzed using a paired non-parametric Wilcoxon test. p-values are indicated on each graph.

    Journal: bioRxiv

    Article Title: LAG3 marks activated but hyporesponsive NK cells

    doi: 10.1101/2024.12.18.629184

    Figure Lengend Snippet: (A) C57BL/6 mice (n=4 for each day) were injected intraperitoneally with 200ug of Poly (I:C) and sacrificed in kinetic. The percentage of Sca1+, LAG3+ and CD69+ splenic NK cells was analyzed by flow cytometry. (B) The percentage of LAG3+ among Sca1+ and Sca1-NK cells on Day2 was analyzed by flow cytometry (n=5). (C-G) C57BL/6 mice (n=2 for each experiment) were injected intraperitoneally with 200ug of Poly (I:C) and sacrificed on Day2. Splenic NK cells were stained for the phospho-epitope pS6 Ser235/236 (C) and for pAkt S473 (D). Overlays of representative histograms are shown (left panels). The MFI values are indicated on the graph and represented on the right panel for each individual, n=6, 3 experiments in total. (E) NK cells were stained with Mitotracker Green and CellRox. The MFI values for each mouse (n=7, 3 experiments in total) for the analyzed marker are represented. (F) The MFI of CD98 (left) or CD71 (right) was determined by flow cytometry on LAG3+ and LAG3-NK cells (n=3). Due to the small sample size, we did not calculate p-values. (G) SSC-A and FSC-A parameters on NK cells were measured by flow cytometry in LAG3- and LAG3+ NK cells (n=5). (H) Splenic NK cells from C57BL/6 mice (n=6, 3 experiments) were isolated, stained with Cell Trace Violet (CTV) and cultured with 100ng/mL of IL-15 for 3 days. The MFI of CTV was analyzed by flow cytometry and the overlays of representative histograms are shown (left panel). The percentage of divided NK cells for each individual was calculated using the proliferation modeling tool on the FlowJo software. All the data were analyzed using a paired non-parametric Wilcoxon test. p-values are indicated on each graph.

    Article Snippet: Isolated NK cells were stained with 0.6µM Invitrogen CellTrace TM Violet (CTV) Cell Proliferation Kit according to manufacturer’s instructions.

    Techniques: Injection, Flow Cytometry, Staining, Marker, Isolation, Cell Culture, Software

    Journal: iScience

    Article Title: Combined TLR2/TLR4 activation equip non-mucosal dendritic cells to prime Th1 cells with gut tropism

    doi: 10.1016/j.isci.2024.111232

    Figure Lengend Snippet:

    Article Snippet: The cells from the cultures with DCs stimulated with MPLA alone were stained with CFSE (CellTrace CFSE Cell Proliferation Kit, for flow cytometry; Invitrogen, #C34554) and the cells from MPLA+P3C activated cultures were stained with CTV (CellTrace Violet Cell Proliferation Kit, for flow cytometry; Invitrogen, #C34557).

    Techniques: Recombinant, Lysis, Cell Isolation, Staining, Enzyme-linked Immunosorbent Assay, Software